tag:blogger.com,1999:blog-72601856193724020292024-03-08T10:42:34.555-08:00B is for BiotechAriah_Mackiehttp://www.blogger.com/profile/12421631014130838283noreply@blogger.comBlogger6125tag:blogger.com,1999:blog-7260185619372402029.post-44880371282949004802018-06-20T00:53:00.001-07:002018-06-20T00:53:57.110-07:00The Spookiness of AII'm <strike>scared</strike> concerned about Artificial Intelligence. Its not the Facebook bot election debacle, or Google knowing that I once looked at butt growth pills (I have a booty for lugeing, I guess) that worries me most though.<br />
<br />
What really causes unease goes a lot deeper and its an issue both of economy and human meaning.<br />
<br />
One day, artificial intelligence will do what we do best better than us. When that comes our jobs will disappear in droves. On one side of the coin, we have tech experts popping champagne to the idea of not working. They make it sound so good. We can hang out with our friends, play in a virtual reality, and lose our responsibilities. It almost sounds like paradise.<br />
<br />
On the other hand, we also have the fast and ugly process of people losing jobs (with the emotional devastation, homelessness, and food shortages) and the achingly slow political process of finding a solution for that. If we let it happen, we are going to have to change the way we think of work and livelihood to support the population. We are going to essentially have to become a welfare state to the most extreme extent.<br />
<br />
I find this idea deeply disturbing and unprecedented in a psychological sense. So much of life's meaning gets derived from work in our culture. As progressive as I am (I'm pro the basics for all: healthcare, emergency food, shelter, and water, and education), I'm still in that crowd of people who would rather work to earn luxuries rather than win the lottery. Skill acquisition, my job, and my goals, play an enormous part in my happiness. The payment for it feels like an additional stamp of approval to show that my pursuits are meaningful to other people. If jobs are going to become a thing of the past, my thinking will have to be re-framed.<br />
<br />
If everything was taken care of, would we feel less useful? Would our self-esteem diminish? Would we become (more) depressed? Would we live in a seemingly worry free paradise where the hedonic effect came so quickly we were essentially miserable because we had nothing we had to work for?<br />
<br />
Even if some jobs didn't remain, they would be highly technical. Not everyone is going to work these jobs. Don't those who are being left out deserve the feeling that are contributing to society as well?<br />
<br />
If we look at recent user technology that's developed, we can see some of the harm. Like the rest of my fellow snowflake millennials, I watched social media come and be normalized. When it entered my life, I was far too young to really think of its implications on emotional health.<br />
<br />
Now, I can see the ill effects. I see the lack of reservation screen to screen contact gives me when telling off republicans (not good). I've seen pictures that caused me envy and feelings of inferiority. I've caught myself wondering what social media would think as I go about my daily private life. I've googled whether I'm narcissistic. I've been on Buzzfeed and I've taken their quizzes.<br />
<br />
It would have be nice if these issues had been predicted with the necessary antidotes, plans, and philosophies to handle it, but I suppose new technology rarely comes out with the proper safety protocols.<br />
<br />
People had to die of electrical shock when electricity was first introduced into homes before someone decided to teach people how to not bathe with toasters. Cars were made, then seat belts. Technology pops up. Some people get ran over. Then, society catches up and creates band aids for it.<br />
<br />
You can't stop technology, but could we ever learn to actually take actions against the ill effects of tech before it happens? Where is our seat belt for A.I.?<br />
<br />
<br />
<br />Ariah_Mackiehttp://www.blogger.com/profile/12421631014130838283noreply@blogger.com0tag:blogger.com,1999:blog-7260185619372402029.post-33506056524783251412017-05-17T21:14:00.004-07:002017-05-17T21:14:39.251-07:00The Methods Behind Protein Engineering<div class="p0" style="margin-bottom: 0pt; margin-top: 0pt; text-autospace: ideograph-other;">
<span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-top: 0pt; text-autospace: ideograph-other;">
<span style="font-size: 13.5pt;">Rather than creating </span><span style="font-size: 13.5pt; font-style: italic;">de novo</span><span style="font-size: 13.5pt;"> structures out of thin air (like a greek God), protein engineering usually consists of slightly mutating natural proteins to give them more desired properties.</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-top: 0pt; text-autospace: ideograph-other;">
<span style="font-size: 13.5pt;"><br /></span><span style="font-size: 13.5pt;">But what is meant by mutating </span><span style="font-size: 13.5pt;">a</span><span style="font-size: 13.5pt;">nd what is the desired result?</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-top: 0pt; text-autospace: ideograph-other;">
<span style="font-size: 13.5pt;"></span><br /><span style="font-size: 13.5pt;"></span><span style="font-size: 13.5pt;">We might be getting ahead of ourselves, so let's do a refresher on how proteins work. </span><span style="font-size: 13.5pt;">To understand </span><span style="font-size: 13.5pt;">all of </span><span style="font-size: 13.5pt;">this you first have to know the central dogma of biology. DNA (the longterm code that determines our genetics) is transcribed into RNA (short lived code), which is translated into proteins.</span><span style="font-size: 13.5pt;"> Proteins help build up the structure and carry out the biological functions of organisms</span><span style="font-size: 13.5pt;">.</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-top: 0pt; text-autospace: ideograph-other;">
<span style="font-size: 13.5pt;"></span><br /><span style="font-size: 13.5pt;"></span><span style="font-size: 13.5pt;">A protein is</span><span style="font-size: 13.5pt;"> made up of</span><span style="font-size: 13.5pt;"> one or more polypeptide chain</span><span style="font-size: 13.5pt;"> subunits</span><span style="font-size: 13.5pt;">. Polypeptide chains are made from</span><span style="font-size: 13.5pt;"> linking a choice of twenty</span><span style="font-size: 13.5pt;"> amino acids</span><span style="font-size: 13.5pt;"> (smallish molecules) end-to-end</span><span style="font-size: 13.5pt;">. These subunits fold together </span><span style="font-size: 13.5pt;">under</span><span style="font-size: 13.5pt;"> normal</span><span style="font-size: 13.5pt;"> (normal = physiological, i.e inside the body)</span><span style="font-size: 13.5pt;"> </span><span style="font-size: 13.5pt;">pH </span><span style="font-size: 13.5pt;">and temperature conditions into a speci</span><span style="font-size: 13.5pt;">alized</span><span style="font-size: 13.5pt;"> </span><span style="font-size: 13.5pt;">structure. This specialized structure </span><span style="font-size: 13.5pt;">performs a certain function in a cell or organism. </span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-top: 0pt; text-autospace: ideograph-other;">
<span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-top: 0pt; text-autospace: ideograph-other;">
<span style="font-size: 13.5pt;">Always remember, in </span><span style="font-size: 13.5pt;">B</span><span style="font-size: 13.5pt;">iology, the form of an object is associated with the function of the object. </span><span style="font-size: 13.5pt;">Structure</span><span style="font-size: 13.5pt;"> is everything.</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-top: 0pt; text-autospace: ideograph-other;">
<span style="font-size: 13.5pt;"></span><br /><span style="font-size: 13.5pt;"></span><span style="font-size: 13.5pt;">When building a protein there are 20 amino acids to choose from:</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-left: 21.2500pt; margin-top: 0pt; text-autospace: ideograph-other; text-indent: -21.2500pt;">
<span style="font-size: 13.5pt;">1. </span><span style="font-size: 13.5pt;">Glycine</span><span style="font-size: 13.5pt;">, (Gly, G)</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-left: 21.2500pt; margin-top: 0pt; text-autospace: ideograph-other; text-indent: -21.2500pt;">
<span style="font-size: 13.5pt;">2. </span><span style="font-size: 13.5pt;">A</span><span style="font-size: 13.5pt;">lanine</span><span style="font-size: 13.5pt;"> (Ala, A)</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-left: 21.2500pt; margin-top: 0pt; text-autospace: ideograph-other; text-indent: -21.2500pt;">
<span style="font-size: 13.5pt;">3. </span><span style="font-size: 13.5pt;">V</span><span style="font-size: 13.5pt;">aline</span><span style="font-size: 13.5pt;"> (Val, V)</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-left: 21.2500pt; margin-top: 0pt; text-autospace: ideograph-other; text-indent: -21.2500pt;">
<span style="font-size: 13.5pt;">4. </span><span style="font-size: 13.5pt;">L</span><span style="font-size: 13.5pt;">eucine</span><span style="font-size: 13.5pt;"> (Leu, L)</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-left: 21.2500pt; margin-top: 0pt; text-autospace: ideograph-other; text-indent: -21.2500pt;">
<span style="font-size: 13.5pt;">5. </span><span style="font-size: 13.5pt;">Isoleucine</span><span style="font-size: 13.5pt;"> (Ile, I)</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-left: 21.2500pt; margin-top: 0pt; text-autospace: ideograph-other; text-indent: -21.2500pt;">
<span style="font-size: 13.5pt;">6. </span><span style="font-size: 13.5pt;">Tr</span><span style="font-size: 13.5pt;">yptophan</span><span style="font-size: 13.5pt;"> (Trp, W)</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-left: 21.2500pt; margin-top: 0pt; text-autospace: ideograph-other; text-indent: -21.2500pt;">
<span style="font-size: 13.5pt;">7. </span><span style="font-size: 13.5pt;">P</span><span style="font-size: 13.5pt;">henylalanine</span><span style="font-size: 13.5pt;"> (Phe, F)</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-left: 21.2500pt; margin-top: 0pt; text-autospace: ideograph-other; text-indent: -21.2500pt;">
<span style="font-size: 13.5pt;">8. </span><span style="font-size: 13.5pt;">T</span><span style="font-size: 13.5pt;">yrosine</span><span style="font-size: 13.5pt;"> (Tyr, Y)</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-left: 21.2500pt; margin-top: 0pt; text-autospace: ideograph-other; text-indent: -21.2500pt;">
<span style="font-size: 13.5pt;">9. </span><span style="font-size: 13.5pt;">T</span><span style="font-size: 13.5pt;">hreonine</span><span style="font-size: 13.5pt;"> (Thr, T)</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-left: 21.2500pt; margin-top: 0pt; text-autospace: ideograph-other; text-indent: -21.2500pt;">
<span style="font-size: 13.5pt;">10. </span><span style="font-size: 13.5pt;">S</span><span style="font-size: 13.5pt;">erine</span><span style="font-size: 13.5pt;"> (Ser, S)</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-left: 21.2500pt; margin-top: 0pt; text-autospace: ideograph-other; text-indent: -21.2500pt;">
<span style="font-size: 13.5pt;">11. </span><span style="font-size: 13.5pt;">C</span><span style="font-size: 13.5pt;">ysteine</span><span style="font-size: 13.5pt;"> (Cys, C)</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-left: 21.2500pt; margin-top: 0pt; text-autospace: ideograph-other; text-indent: -21.2500pt;">
<span style="font-size: 13.5pt;">12. </span><span style="font-size: 13.5pt;">M</span><span style="font-size: 13.5pt;">ethionine</span><span style="font-size: 13.5pt;"> (Met, M)</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-left: 21.2500pt; margin-top: 0pt; text-autospace: ideograph-other; text-indent: -21.2500pt;">
<span style="font-size: 13.5pt;">13. </span><span style="font-size: 13.5pt;">G</span><span style="font-size: 13.5pt;">lutamat</span><span style="font-size: 13.5pt;">ic Acid/Glutamate (Glu, E)</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-left: 21.2500pt; margin-top: 0pt; text-autospace: ideograph-other; text-indent: -21.2500pt;">
<span style="font-size: 13.5pt;">14. </span><span style="font-size: 13.5pt;">Gl</span><span style="font-size: 13.5pt;">utamine</span><span style="font-size: 13.5pt;"> (Gln, Q)</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-left: 21.2500pt; margin-top: 0pt; text-autospace: ideograph-other; text-indent: -21.2500pt;">
<span style="font-size: 13.5pt;">15. </span><span style="font-size: 13.5pt;">A</span><span style="font-size: 13.5pt;">rginine</span><span style="font-size: 13.5pt;"> (Arg, R)</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-left: 21.2500pt; margin-top: 0pt; text-autospace: ideograph-other; text-indent: -21.2500pt;">
<span style="font-size: 13.5pt;">16. </span><span style="font-size: 13.5pt;">A</span><span style="font-size: 13.5pt;">spart</span><span style="font-size: 13.5pt;">ic Acid/Aspartate (Asp, D)</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-left: 21.2500pt; margin-top: 0pt; text-autospace: ideograph-other; text-indent: -21.2500pt;">
<span style="font-size: 13.5pt;">17. </span><span style="font-size: 13.5pt;">A</span><span style="font-size: 13.5pt;">sparagine</span><span style="font-size: 13.5pt;"> (Asn, N)</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-left: 21.2500pt; margin-top: 0pt; text-autospace: ideograph-other; text-indent: -21.2500pt;">
<span style="font-size: 13.5pt;">18. </span><span style="font-size: 13.5pt;">L</span><span style="font-size: 13.5pt;">ysine</span><span style="font-size: 13.5pt;"> (Lys, K)</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-left: 21.2500pt; margin-top: 0pt; text-autospace: ideograph-other; text-indent: -21.2500pt;">
<span style="font-size: 13.5pt;">19. </span><span style="font-size: 13.5pt;">Histidine</span><span style="font-size: 13.5pt;"> (His, H)</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-left: 21.2500pt; margin-top: 0pt; text-autospace: ideograph-other; text-indent: -21.2500pt;">
<span style="font-size: 13.5pt;">20. </span><span style="font-size: 13.5pt;">P</span><span style="font-size: 13.5pt;">roline</span><span style="font-size: 13.5pt;"> (Pro, P)</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-top: 0pt; text-autospace: ideograph-other;">
<span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="float: left; margin-right: 1em; text-align: left;"><tbody>
<tr><td style="text-align: center;"><img height="133" src="https://upload.wikimedia.org/wikipedia/commons/7/74/Alpha-amino-acid-general-2D.png" style="margin-left: auto; margin-right: auto;" width="200" /></td></tr>
<tr><td class="tr-caption" style="text-align: center;">The general form for an amino acid.</td></tr>
</tbody></table>
<div class="p0" style="margin-bottom: 0pt; margin-top: 0pt; text-autospace: ideograph-other;">
<span style="font-size: 13.5pt;">An amino acid has four basic parts surrounding a central alpha carbon: an </span><span style="font-size: 13.5pt;">amine group, a carboxyl group, a Hydrogen, and a wild card R group</span><span style="font-size: 13.5pt;">. </span><span style="font-size: 13.5pt;">The carboxylic group</span><span style="font-size: 13.5pt;"> of one amino acid </span><span style="font-size: 13.5pt;">link</span><span style="font-size: 13.5pt;">s</span><span style="font-size: 13.5pt;"> with a following amino acids' amide group to form a peptide </span><span style="font-size: 13.5pt;">bond</span><span style="font-size: 13.5pt;">.</span><span style="font-size: 13.5pt;"> A series of these peptide bonds forms a peptide chain.</span><span style="font-size: 13.5pt;"> This is a protein's </span><span style="font-size: 13.5pt; font-weight: bold;">primary structure.</span><span style="font-size: 13.5pt; font-weight: bold;"> </span><span style="font-size: 13.5pt;">If our protein was a piece of clothing, we might say that its primary structure is a single strand of thread that makes up the clothing.</span><span style="font-size: 13.5pt; font-weight: bold;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-top: 0pt; text-autospace: ideograph-other;">
<span style="font-size: 13.5pt; font-weight: bold;"><o:p></o:p></span></div>
<table cellpadding="0" cellspacing="0" class="tr-caption-container" style="float: left; text-align: left;"><tbody>
<tr><td style="text-align: center;"><a href="https://upload.wikimedia.org/wikipedia/commons/7/75/Alpha_helix.png" imageanchor="1" style="clear: left; margin-bottom: 1em; margin-left: auto; margin-right: auto;"><img border="0" height="320" src="https://upload.wikimedia.org/wikipedia/commons/7/75/Alpha_helix.png" width="136" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">An Alpha Helix</td></tr>
</tbody></table>
<div class="p0" style="margin-bottom: 0pt; margin-top: 0pt; text-autospace: ideograph-other;">
<span style="font-size: 13.5pt;">Th</span><span style="font-size: 13.5pt;">e</span><span style="font-size: 13.5pt;"> chain will then fold into </span><span style="font-size: 13.5pt;">what is called</span><span style="font-size: 13.5pt;"> </span><span style="font-size: 13.5pt; font-weight: bold;">secondary structure</span><span style="font-size: 13.5pt;">.</span><span style="font-size: 13.5pt;"> </span><span style="font-size: 13.5pt;">The most </span><span style="font-size: 13.5pt;">common</span><span style="font-size: 13.5pt;"> secondary structure </span><span style="font-size: 13.5pt;">types </span><span style="font-size: 13.5pt;">are the </span><span style="font-size: 13.5pt; font-weight: bold;">alpha helix</span><span style="font-size: 13.5pt;"> and the </span><span style="font-size: 13.5pt; font-weight: bold;">beta sh</span><span style="font-size: 13.5pt; font-weight: bold;">eet. </span><span style="font-size: 13.5pt;">The alpha helix looks like a right handed stair case that twists upwards by 3.5 residues per turn. The R groups of each residue (amino acid) stick out the sides of this long winding twist.</span><span style="font-size: 13.5pt;"> The beta sheets look like wavy sheets stacked on top of each other. </span><span style="font-size: 13.5pt;">For our clothing metaphor, the secondary structure might be small knots and loops in the thread</span><span style="font-size: 13.5pt;">.</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-top: 0pt; text-autospace: ideograph-other;">
<span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><img alt="Image result for beta sheet" height="200" src="https://upload.wikimedia.org/wikipedia/commons/thumb/c/c9/Alpha_sheet_bonding_schematic-color.svg/1004px-Alpha_sheet_bonding_schematic-color.svg.png" style="margin-left: auto; margin-right: auto;" width="196" /></td></tr>
<tr><td class="tr-caption" style="text-align: center;">A beta sheet</td></tr>
</tbody></table>
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><img alt="Image result for beta sheet" height="207" src="https://upload.wikimedia.org/wikipedia/commons/b/b8/Beta-meander1.png" style="margin-left: auto; margin-right: auto;" width="320" /></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Another representation of a beta sheet.</td></tr>
</tbody></table>
<div class="p0" style="margin-bottom: 0pt; margin-top: 0pt; text-autospace: ideograph-other;">
<span style="font-size: 13.5pt;">The </span><span style="font-size: 13.5pt; font-weight: bold;">tertiary</span><span style="font-size: 13.5pt;"> structure is the overall 3D globular structure of a protein. This is the point where proteins really start looking like, well, proteins. There is a 3D structure. For our clothing metaphor, you can imagine the tertiary structure is the formation of a pant leg. Some proteins only go up to this tertiary structure.</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><img alt="Image result for tertiary structure" height="176" src="https://upload.wikimedia.org/wikipedia/commons/2/2d/I-Tasser_Predicted_Tertiary_Structure_1.png" style="margin-left: auto; margin-right: auto;" width="200" /></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Tertiary Structure</td></tr>
</tbody></table>
<div class="p0" style="margin-bottom: 0pt; margin-top: 0pt; text-autospace: ideograph-other;">
<span style="font-size: 13.5pt;"><br /></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-top: 0pt; text-autospace: ideograph-other;">
<span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<div class="p0" style="margin-bottom: 0pt; margin-top: 0pt; text-autospace: ideograph-other;">
<span style="font-size: 13.5pt;">Sometimes, but not always, tertiary structure pieces made of different peptide chains come together to form </span><span style="font-size: 13.5pt; font-weight: bold;">quaternary</span><span style="font-size: 13.5pt;"> structure. You can think of this as pant legs and other pieces coming together to make a pair of functional jeans. The pieces are from a different strand of thread (a different peptide chain), but the parts work together.</span><span style="font-size: 13.5pt;"><o:p></o:p></span></div>
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><img alt="Image result for tertiary structure" height="337" src="https://upload.wikimedia.org/wikipedia/commons/2/26/225_Peptide_Bond-01.jpg" style="margin-left: auto; margin-right: auto;" width="400" /></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Hemoglobin is protein with Quaternary structure.</td></tr>
</tbody></table>
<div class="p0" style="margin-bottom: 0pt; margin-top: 0pt; text-autospace: ideograph-other;">
<span style="font-size: 13.5pt;"><br /></span></div>
<div>
</div>
<div>
<br /></div>
<div>
<br /></div>
<div>
Sources:</div>
<div>
https://upload.wikimedia.org/wikipedia/commons/7/74/Alpha-amino-acid-general-2D.png\<br />
https://upload.wikimedia.org/wikipedia/commons/7/75/Alpha_helix.png<br />
https://upload.wikimedia.org/wikipedia/commons/thumb/c/c9/Alpha_sheet_bonding_schematic-color.svg/1004px-Alpha_sheet_bonding_schematic-color.svg.png<br />
https://upload.wikimedia.org/wikipedia/commons/b/b8/Beta-meander1.png<br />
https://upload.wikimedia.org/wikipedia/commons/2/2d/I-Tasser_Predicted_Tertiary_Structure_1.png<br />
https://upload.wikimedia.org/wikipedia/commons/2/26/225_Peptide_Bond-01.jpg<br />
<br />
<br /></div>
<div>
</div>
Unknownnoreply@blogger.com0tag:blogger.com,1999:blog-7260185619372402029.post-33268365173183407582017-03-19T15:19:00.001-07:002017-03-24T15:41:44.364-07:00Top 10 Bioinformatics Resources for Students to Get Started<h3>
<ol>
<li> <a href="https://www.ncbi.nlm.nih.gov/books/NBK3831/">The NCBI Help Manual</a></li>
<ul>
<li>for using BLAST, PubMed, and Entrez</li>
</ul>
<li><a href="http://rosalind.info/problems/locations/">Rosalind</a></li>
<ul>
<li>for hands on python learning and practice bioinformatics problems</li>
</ul>
<li><a href="https://www.tutorialspoint.com/python/index.htm">Tutorials Point for Python</a></li>
<ul>
<li>all the basics of python</li>
</ul>
<li><a href="https://github.com/zhiwehu/Python-programming-exercises/blob/master/100%2B%20Python%20challenging%20programming%20exercises.txt">100 + Challenging Python Exercises</a></li>
<ul>
<li>To master the python language in practice</li>
</ul>
<li><a href="http://ryanstutorials.net/linuxtutorial/">Ryans Tutorials on Linux Systems</a></li>
<ul>
<li>for starting on linux</li>
</ul>
<li><a href="https://files.fosswire.com/2007/08/fwunixref.pdf">Linux CheatSheet</a></li>
<ul>
<li>an easy reference</li>
</ul>
<li><a href="https://www.amazon.com/Introduction-Bioinformatics-Arthur-Lesk/dp/0199208042"> Introduction into Bioinformatics by Lesk</a> (pick most current version you can afford)</li>
<ul>
<li>to understand some of the fundamental algorithms used in bioinformatics</li>
</ul>
<li><a href="https://xlinux.nist.gov/dads/"> The Dictionary of Algorithms and Data Structures</a></li>
<li><a href="http://www.bogotobogo.com/Algorithms/algorithms.php">The Blog that Plays Music and Teaches You Algorithms</a></li>
<ul>
<li>learn what many algorithms and data structures are for so you can use them when needed for a project</li>
</ul>
<li><a href="https://github.com/open-source-society/bioinformatics">A Guide to Bioinformatics Self Learning</a></li>
<ul>
<li>MIT based courses on Bioinformatics</li>
</ul>
</ol>
<div>
<br /></div>
</h3>
Ariah_Mackiehttp://www.blogger.com/profile/12421631014130838283noreply@blogger.com0tag:blogger.com,1999:blog-7260185619372402029.post-45660867678873158092017-03-19T14:22:00.001-07:002017-03-19T14:22:22.074-07:00The Basics of Extracting DNA from Liquid Media (Old School)Today's focus is on isolating DNA from bacteria<i>.</i><br />
<br />
I'll outline the basic steps, sans detailed numbers.<br />
<br />
Note: This overview uses phenol and chloroform, which are both dangerous substances. Only work with these substances if you are trained, have the proper safety gear ( gloves, lab coat, safety googles and a fume hood), emergency resources, and if there is someone else in the lab with you. (Never work alone.)<br />
<br />
<b>Step 1: Spin</b><br />
<b><br /></b>
Starting with a solution of cells in media derived from one colony, spin the cells in a centrifuge till the pellet is at the bottom of the tube. The fluid that remains is called the supernatant. Discard it without disrupting your pellet. Don't feel guilty.<br />
<br />
<b>Step 2: Rip Cells Apart with a Lysis Buffer</b><br />
Mix a few milliliters (say.. 10 ml) of Lysis Buffer:<br />
<u>100 mM EDTA, </u><br />
<u>10 mM Tris (pH 7.5)</u><br />
<u> and 1% SDS. </u><br />
<br />
Add a roughly equal amount of lysis buffer as you have bacteria. Using a pestle and vortexor, grind up your cells into they are completely lysed and stop begging for mercy. Spin this solution down and keep the supernatant.<br />
<b><br /></b>
<div style="line-height: 1.0500pt; margin-bottom: 0pt; margin-top: 0pt; text-autospace: ideograph-other;">
<b>Step 3: "Coagulate" Proteins and Start Isolating Nucleic Acids with Phenol</b></div>
<br />
Now add an equal volume of phenol as your supernatant. Mix by inversion a couple of times. Spin solution. Pipette the top layer into a new tube. Discard the rest.<br />
<br />
<b>Step 4: Use 1:1 Phenol Chloroform</b><br />
<br />
Add 1:1 phenol chloroform in equal volume to solution. Mix again by inversion. Spin. Then pipette off top layer to keep.<br />
<br />
<b>Step 5: Repeat One More time, but now with 100% Chloroform</b><br />
Add chloroform in equal volume solution. Mix again by inversion. Spin. Pipette off top layer to keep.<br />
<br />
<b>Step 6: <span style="font-family: Arial; font-size: 11pt;">Add 7.5 M NH4OAc</span></b><br />
<div class="p0" style="line-height: 1.0500pt; margin-bottom: 0pt; margin-top: 0pt; text-autospace: ideograph-other;">
<span style="font-family: Arial; font-size: 11pt;"><o:p></o:p></span></div>
Pipette 1/2 of your solution volume of 7.5 M NH4OAc to your solution.<br />
<br />
<b>Step 7: Add ethanol</b><br />
<br />
Add enough ethanol to reach a 66% ethanol solution. Invert a few times.<br />
<b><br /></b>
<b>Step 8: Wait patiently</b><br />
<b><br /></b>
Wait about 10 minutes with your solution at room temperature (It is recommended for steps that you do things on ice to prevent degrading DNA accept for when re-suspending your DNA pellet at the end.) Twiddle your thumbs.<br />
<br />
<b>Step 9: Spin Spin Spin</b><br />
<br />
Spin sample until your DNA pellet is on the side of the tube. Decant ethanol without disrupting or moving your pellet.<br />
<b><br /></b>
<b>Step 10: Wash with 70% ethanol.</b><br />
<br />
Add 70% ethanol. Centrifuge and then decant supernatant carefully without harming your pellet.<br />
<br />
<b>Step 11: Dry and Wet Again</b><br />
<b><br /></b>
Let ethanol evaporate off the tube. Then re-suspend DNA in 20-100 ul of TE buffer or distilled water lab grade water. Give the DNA time to dissolve into solution.<br />
<br />
<br />
<br />
<br />
<br />
Once the DNA is isolated, check the concentration using a Nanodrop. A Nanodrop is a spectrophotometer that can measure the concentration of DNA, protein, or RNA, using a beam of light and Beer's law. In essence, the Nanodrop measures the absorbance of light caused by a small droplet of your solution. Then it uses an equation to calculate the concentration of your substance based on how much light got absorbed by the particulates in your droplet.<br />
<br />
<br />
Resource and For Further Reading: <a href="http://palumbi.stanford.edu/SimpleFoolsMaster.pdf"> http://palumbi.stanford.edu/SimpleFoolsMaster.pdf</a><br />
<br />
<br />
<br />Unknownnoreply@blogger.com0tag:blogger.com,1999:blog-7260185619372402029.post-62808752181540177882017-01-17T22:49:00.002-08:002017-01-19T22:22:22.090-08:00Easing Aging by Yamanaka Factors?<h2>
Article Highlight Reel of the Week:</h2>
<span style="font-family: "arial" , "helvetica" , sans-serif;"><br /></span>
<span style="font-family: "arial" , "helvetica" , sans-serif;">This week I'm focusing on the article: "<span style="background-color: white;"><a href="http://www.cell.com/cell/abstract/S0092-8674(16)31664-6?_returnURL=http%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0092867416316646%3Fshowall%3Dtrue">In Vivo Amelioration of Age-Associated Hallmarks by Partial Reprogramming</a>" which was published in Cell recently. </span></span><br />
<span style="background-color: white;"><span style="font-family: "arial" , "helvetica" , sans-serif;"><br /></span></span>
<br />
<h3>
<span style="background-color: white;"><b><span style="font-family: "arial" , "helvetica" , sans-serif;">Here is the background you need to know to understand this paper:</span></b></span></h3>
<span style="font-family: "arial" , "helvetica" , sans-serif;"><br /></span>
<span style="font-family: "arial" , "helvetica" , sans-serif;"><b>Stem cells</b> are a type of cell that can develop into different types of cells. Put simply, a pluripotent stem cell has a lot of potential to be just about anything when it grows up. Like a child pondering what he or she can be, it has many paths it can take in development before it turns into its final adult career*. However, as it differentiates and goes down the path to maturity, its options of what it can be dwindles. Once it is a mature cell, it can't revert back to its pluripotent state (without some help from scientists).</span><br />
<span style="font-family: "arial" , "helvetica" , sans-serif;"><br /></span>
<span style="font-family: "arial" , "helvetica" , sans-serif;">The </span><b style="font-family: Arial, Helvetica, sans-serif;">potency</b><span style="font-family: "arial" , "helvetica" , sans-serif;"> of a stem cell refers to how many possible types of cell your stem cell could become. If the maturing cell is <b>unipotent</b>, that means it must be at stage of differentiation where it can only become one cell type. If it is </span><b style="font-family: Arial, Helvetica, sans-serif;">pluripotent</b><span style="font-family: "arial" , "helvetica" , sans-serif;">, it can become just about any cell type in the body.</span><br />
<span style="font-family: "arial" , "helvetica" , sans-serif;"><br /></span>
<span style="font-family: "arial" , "helvetica" , sans-serif;">There are three sources of stem cells known. Those are: <b>adult stem cells </b>(which you have right now), <b>embroyonic stem cells</b> (which are derived from a 5 day old blastocyst), and i<b>nduced pluripotent stem cells (iPSC)</b> which are the subject of this paper.</span><br />
<span style="font-family: "arial" , "helvetica" , sans-serif;"><br /></span>
<span style="font-family: "arial" , "helvetica" , sans-serif;"><b><a href="https://www.nobelprize.org/nobel_prizes/medicine/laureates/2012/yamanaka-facts.html">Shinya Yamanaka</a></b> won the Nobel Prize in medicine in 2012 for discovering four genes that when expressed can take a mature cell back to its pluripotent stem cell stage. The problem is these cells, though they appear to be brought back to a state of youth, can grow into cancer. So, the simple idea of applying Yamanaka factors haphazardly just doesn't work.</span><br />
<h3>
<br /><span style="background-color: white;"><span style="font-family: "arial" , "helvetica" , sans-serif;">Here are the highlight discoveries:</span></span></h3>
<span style="font-family: "arial" , "helvetica" , sans-serif;"><br /></span>
<span style="font-family: "arial" , "helvetica" , sans-serif;">Scientists from the <a href="http://www.salk.edu/">Salk Insitute</a> figured out that they could turn the Yamanka genes on and off before cells reached a totally pluripotent state (in which the cells could turn to cancer).</span><br />
<span style="font-family: "arial" , "helvetica" , sans-serif;"><br /></span>
<span style="font-family: "arial" , "helvetica" , sans-serif;">The way they did this was by designing mice with Yamanaka genes which could be activated or repressed when the mice ingest a substance in their water. They activated Yamanaka genes for two days and repressed them for five in cyclic fashion.</span><br />
<span style="font-family: "arial" , "helvetica" , sans-serif;"><br /></span>
<span style="font-family: "arial" , "helvetica" , sans-serif;">Mice who had </span><a href="http://www.webmd.com/children/guide/progeria#1" style="font-family: Arial, Helvetica, sans-serif;">progeria</a><span style="font-family: "arial" , "helvetica" , sans-serif;">, which is a disease that causes one to age quickly, lived 30% longer than their average lifespan. Normal mice showed less aging characteristics. However, this beneficial effect did not last long after treatment stopped.</span><br />
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="float: left; margin-right: 1em; text-align: left;"><tbody>
<tr><td style="text-align: center;"><img alt="Image result for progeria" height="258" src="https://upload.wikimedia.org/wikipedia/commons/6/6e/Hutchinson-Gilford_Progeria_Syndrome.png" style="margin-left: auto; margin-right: auto;" width="320" /></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Child With Progeria</td></tr>
</tbody></table>
<span style="font-family: "arial" , "helvetica" , sans-serif;"><br /></span>
<span style="font-family: "arial" , "helvetica" , sans-serif;"><b>So, in brief, this study showed that cyclicly turning on Yamanka factors appears to slow aging in mice.</b></span><br />
<span style="font-family: "arial" , "helvetica" , sans-serif;"><b><br /></b></span>
<br />
<h3>
<span style="font-family: "arial" , "helvetica" , sans-serif;">Why it's a Cool Paper:</span></h3>
<div>
<span style="font-family: "arial" , "helvetica" , sans-serif;"><br /></span></div>
<div>
<span style="font-family: "arial" , "helvetica" , sans-serif;">The problem of pluripotent stem cells forming cancer cells has been a key issue of applying iPScs in human health care.</span></div>
<div>
<span style="font-family: "arial" , "helvetica" , sans-serif;"><br /></span></div>
<div>
<span style="font-family: "arial" , "helvetica" , sans-serif;">Even though we won't be doing gene editing on people to replicate this experiment, this study can be a springboard to study how <b>epigenetics</b> (a subfield of genetics which studies how genes get turned on and off at certain times for certain reasons) relates to aging. Who knows? Perhaps from this knowledge a sensible antiaging therapy could develop in the future. </span></div>
<div>
<span style="font-family: "arial" , "helvetica" , sans-serif;"><br /></span></div>
<div>
<span style="font-family: "arial" , "helvetica" , sans-serif;"><i>But no antiaging water just yet...</i></span></div>
<span style="font-family: "arial" , "helvetica" , sans-serif;"><br /></span>
<span style="font-family: "arial" , "helvetica" , sans-serif;"><br /></span>
<span style="font-family: "arial" , "helvetica" , sans-serif;"><br /></span>
<span style="font-family: "arial" , "helvetica" , sans-serif;"><br /></span>
<br />
<div>
<span style="font-family: "arial" , "helvetica" , sans-serif;">Additional Sources and Reading:</span></div>
<div>
<span style="font-family: "arial" , "helvetica" , sans-serif;"><a href="http://www.smithsonianmag.com/innovation/have-scientists-found-way-to-actually-reduce-effects-aging-180961808/">http://www.smithsonianmag.com/innovation/have-scientists-found-way-to-actually-reduce-effects-aging-180961808/</a></span></div>
<div>
<span style="font-family: "arial" , "helvetica" , sans-serif;">Image Source: <i style="background-color: #f8f9fa; color: #555555; line-height: 24.48px;">The Cell Nucleus and Aging: Tantalizing Clues and Hopeful Promises.</i><span style="background-color: #f8f9fa; color: #555555; line-height: 24.48px;"> Scaffidi P, Gordon L, Misteli T. PLoS Biology Vol. 3/11/2005, e395 </span><a class="extiw" href="https://dx.doi.org/10.1371/journal.pbio.0030395" style="background: none rgb(248, 249, 250); color: #0b0080; line-height: 24.48px; text-decoration: none;" title="doi:10.1371/journal.pbio.0030395">doi:10.1371/journal.pbio.0030395</a></span></div>
<div>
<span style="font-family: "arial" , "helvetica" , sans-serif;"><br /></span></div>
<div>
<span style="font-family: "arial" , "helvetica" , sans-serif;"><br /></span></div>
<div>
<span style="font-family: "arial" , "helvetica" , sans-serif;">*</span><span style="font-family: "arial" , "helvetica" , sans-serif;">(ignore the fact that most people have many careers in their lifetime for metaphor sake)</span></div>
<br />
<span style="background-color: white; font-family: "arial" , sans-serif;"><br /></span>Unknownnoreply@blogger.com0tag:blogger.com,1999:blog-7260185619372402029.post-23682761724626081292016-04-27T23:47:00.001-07:002016-04-27T23:55:17.006-07:00How to Make Starting Lab Work Easier: Part 1<div style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px; margin-bottom: 1.1em; margin-top: 1.1em;">
I'm chronicling my adventures in the lab to bring what it's like to work as a new scientist to the forefront. </div>
<h3 style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 1.2em; letter-spacing: -1px; line-height: 1.2em;">
On pipettes</h3>
<div style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px; margin-bottom: 1.1em; margin-top: 1.1em;">
<i>You are given a long pointy instrument that measures to the precision of microliters. You are told to transfer tiny rain droplets of what looks like spit. Your hands have the tendency to shake. The gloves on your hands feel weird like there is less hold or contact.</i></div>
<h3 style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 1.2em; letter-spacing: -1px; line-height: 1.2em;">
</h3>
<h3 style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 1.2em; letter-spacing: -1px; line-height: 1.2em;">
<span style="font-weight: normal;">The naïve biotech wannabe must first face the task of learning to pipette. In brief, a pipette is a glorified turkey baster. During the course of a lab day, one has to choose between a p10, p20, p200, and p1000. The numbers signify the largest amount of microliters that pipette can handle. I don't know what the "p" stands for. Probably, pipette.</span></h3>
<div style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px; margin-bottom: 1.1em; margin-top: 1.1em;">
<br /></div>
<div style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px; margin-bottom: 1.1em; margin-top: 1.1em;">
Pipettes are good at sucking and spitting out. I know that's not flattering, but it will be your constant companion. </div>
<div style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px; margin-bottom: 1.1em; margin-top: 1.1em;">
<span style="font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px;">So, you have to treat it right, but that can be a challenge. There's a lot to mess up like: </span></div>
<ol style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px;">
<li style="margin-bottom: 1em;">Touching the tip of the pipette to any surface in the lab- That's how you get contamination in your samples.</li>
<li style="margin-bottom: 1em;">Holding the pipette horizontally- Keep the pipette tip pointed down. Gravity rules all. Your pipetted material will flow up into the pipette. Then, it will be dirty the next time someone else uses it. I'm no clean freak. I don't take messiness seriously, except for in the lab. In that environment, being a little unsanitary could mean a project delay of months and wasted money (thousands). What if someone's working on a cure for HIV and you delay that? Sad. It could also cause a biohazard.</li>
<li style="margin-bottom: 1em;"> Get a clean pipette tip every time you pipette into a new container. Don't double dip.</li>
<li style="margin-bottom: 1em;"> When ejecting a pipette tip, (you push a fancy ejection button) don't put it inside the ejection container. Keep it a few inches above. It's cleaner that way.</li>
</ol>
<div style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px; margin-bottom: 1.1em; margin-top: 1.1em;">
<br /></div>
<div style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px; margin-bottom: 1.1em; margin-top: 1.1em;">
<br /></div>
<h3 style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px; margin-bottom: 1.1em; margin-top: 1.1em;">
Basic Use: MEASURE, STAB, SUCTION, EXPULSION</h3>
<div style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px; margin-bottom: 1.1em; margin-top: 1.1em;">
Slide a rotating knob to <strong>measure</strong> your amount in microliters.</div>
<div style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px; margin-bottom: 1.1em; margin-top: 1.1em;">
<strong>Stab</strong> pipette into a matching sized tip.</div>
<div style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px; margin-bottom: 1.1em; margin-top: 1.1em;">
Press plunger down, and stop when you first meet resistance.</div>
<div style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px; margin-bottom: 1.1em; margin-top: 1.1em;">
Place in the sample and remove thumb pressure from the plunger.</div>
<div style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px; margin-bottom: 1.1em; margin-top: 1.1em;">
Release your sample by pressing the plunger down once again to the second click. </div>
<div style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px; margin-bottom: 1.1em; margin-top: 1.1em;">
<br /></div>
<div style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px; margin-bottom: 1.1em; margin-top: 1.1em;">
Getting used to the feeling of when you're pipetting to the first or second click sometimes takes time. You need it for accuracy, though.</div>
<h3 style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px; margin-bottom: 1.1em; margin-top: 1.1em;">
Quick tips:</h3>
<div style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px; margin-bottom: 1.1em; margin-top: 1.1em;">
Practice pipetting droplets of water on wax paper until you get a feel for the instrument.</div>
<div style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px; margin-bottom: 1.1em; margin-top: 1.1em;">
<span style="font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px;">Pipette your cheap liquids first. It is ideal to start with something like water and to destroy a water only sample than to start with an expensive enzyme and mess that sample up.</span></div>
<div style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px; margin-bottom: 1.1em; margin-top: 1.1em;">
<span style="font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px;">If the material you are working with is viscous, dip the pipette tip only near the surface. This prevents extra material from sticking to the outside of the pipette.</span></div>
<div style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px; margin-bottom: 1.1em; margin-top: 1.1em;">
<span style="font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px;">Try to place the sample you are transferring into the bottom of the tube. </span></div>
<div style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px; margin-bottom: 1.1em; margin-top: 1.1em;">
Avoid blowing bubbles.</div>
<div style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px; margin-bottom: 1.1em; margin-top: 1.1em;">
Shaking hands can get better with increased comfort and the loss of newbie's nerves . You can stabilize your arms further by putting both elbows on a table and holding your wrist with your nonpipetting hand.</div>
<div style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px; margin-bottom: 1.1em; margin-top: 1.1em;">
Always think a second ahead about what container you need to open just before you pipette up liquid. Close containers shortly after to minimize contamination.</div>
<div style="color: #0d0d0d; font-family: Merriweather, Georgia, 'Times New Roman', serif; font-size: 19.2px; letter-spacing: -0.7px; line-height: 28.8px; margin-bottom: 1.1em; margin-top: 1.1em;">
<br /></div>
Unknownnoreply@blogger.com0